| Property | Value |
|---|---|
| lemon:value | 
“The HCMV DNA was detected using HCMV1 ( 5 ' cct agt gtg gat gac cta cgg gcc a ) and HCMV2 ( 5 ' cag aca cag tgt cct ccc gct cct c ) primers producing 249 bp long amplicon and the DNA of HHV6 was amplified with specific primer pair HP0 ( 5 ' ccg caa tcg aat cca cct agc gg ) and HP4 ( 5 ' gtg aga acg gat tcg aac agt gct g ) yielding 440 bp product [ 23 24 ] . All amplifications were carried out with 20 pmol of each primer in 2 mM solution of MgCl 2 , 2 U of Tag Special DNA polymerase ( Biovendor , Czech Republic ) , 0.3 mM of each dNTPs , 10 × reaction buffer and 1 ?g of isolated DNA according to the following conditions : 96°C for 4 min , ( 94°C for 10 sec , 58°C for 10 sec , 72°C for 20 sec ) 36 times , and final extension at 72°C for 2 min .”
|
| uby:exampleType | category:senseInstance |
| Property | Value |
|---|---|
| Is lemon:example of | FN_Sense_11498 More... |
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